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KMID : 0364820100460040326
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Korean Journal of Microbiology
2010 Volume.46 No. 4 p.326 ~ p.333
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Characterization of Antimicrobial Resistance Patterns and Integrons of Nontyphoid Salmonella Isolates from Infants in Seoul
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Jin Young-Hee
Kim Jin-Ah
Jung Ji-Hun
Jeon Su-Jin
Lee Jae-Kyoo
Oh Young-Hee
Han Ki-Young
Lee Young-Ki
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Abstract
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A total of 105 nontyphoid Salmonella isolated from infants in Seoul from 2003 to 2009 was investigated for their serotype, antimicrobial resistance, characterization of integron, and the patterns of Pulsed-field gel electrophoresis (PFGE). Eighteen serotypes were detected in 105 isolates, and the two most common serotypes were S. Enteritidis (47.6%) and Montevideo (15.2%). Among the Salmonella serovars, a high level of antimicrobial resistance was found to ampicilin (60%), tetracycline
(46.7%), streptomycin (35.2%) and nalidixic acid (28.6%). In the multi-drug resistance patterns, the predominant patterns were only nalidixic acid (15.7%), ampicillin-ampicillin/sulbactam-tetracycline (14.5%), and ampicillin-streptomycin-chloramphenicol-tetracycline (10.8%). PCR and DNA sequencing analysis revealed the presence of class 1 integron in 20 isolates (19%). Of the class 1 integron positive isolates 20% harboured the integron-associated gene cassettes : aadA2, blaP1, dfr17-aadA5, dfrA12- aadA2, and aadA7. PFGE was carried out to examine the genetic relatedness among S. Enteritidis isolates. Except for three strains, fifty strains were divided by three pulsotypes.
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KEYWORD
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antimicrobial resistance, gene cassette, integron, nontyphoid Salmonella, PFGE
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